Coding

Part:BBa_K3147009:Design

Designed by: Thomas Bessede   Group: iGEM19_Montpellier   (2019-10-14)

TEV protease fused to anti-sfGFP VHH with TEV-cleavable maltose binding protein


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 77
    Illegal BsaI.rc site found at 2398
    Illegal SapI.rc site found at 1541
    Illegal SapI.rc site found at 1889


The TEV protease is a highly specific cysteine protease of the tabacco ech virus sequence.

The protease is highly specific for the consensus sequence for its cleavage site which is ENLYFQ\G (Part:BBa_ J18918) with \ indicating cleaved peptide binding.